Genetic disorders of ketone metabolism such as succinyl-CoA:3-oxoacid CoA transferase deficiency or β-thioketolase deficiency were not present as these are usually detected in childhood.

An inability to detect the regeneration of acetyl-CoA from succinyl-CoA during autotrophic growth of M. sedula led Fuchs and co-workers to propose a modified form of this pathway in this autotroph.

The reaction catalyzed by succinyl-CoA synthetase (in which GTP synthesis occurs) is an example of substrate-level phosphorylation.