Enzymatic recycling has gained traction in recent years as a greener alternative to traditional plastic recycling techniques, ...

There is mounting evidence that enzyme diffusivity is enhanced when the enzyme is catalytically active. Here, using superresolution microscopy [stimulated emission-depletion fluorescence correlation ...

Substrate concentration As substrate concentration increases, the rate of an enzyme-catalysed reaction initially rises as more substrate molecules are available to bind to enzyme active sites. However ...

Glycans can regulate their own biosynthesis by modifying enzyme activity Peer-Reviewed Publication Institute for Glyco-core Research (iGCORE), Tokai National Higher Education and Research System ...

The partially purified fraction showing highest specific activity was characterized by varying the parameters that influence enzyme activity. Substrate concentration The effect of assay substrate ...

How to Interpret Lineweaver-Burk Plots Lineweaver-Burk plots are widely used in enzyme kinetics studies due to their simplicity and ability to provide valuable information. The slope of the line in a ...

4. Progress Curve Analysis Monitoring the progress of an enzymatic reaction as it proceeds from start to finish can provide valuable information about enzyme activity. By measuring the change in ...

Enzyme kinetics involves the measurement of the rate at which chemical reactions that are catalyzed by enzymes occur. Knowledge about the kinetics of an enzyme can reveal useful information about ...

Free FB substrate was used at 1.1 μM final concentration. The complex substrate C3bB was prepared from FB and C3b at 1.1 and 1.3 μM final concentrations, respectively. A slight excess of C3b was used ...

Enzyme reactions are always dictated by the ratio of an enzyme to its substrate in the reaction concentration. In the example of lipases (an enzyme that breaks down fats), it needs a substrate (fats) ...