Environmental Microbiome Engineering and Biotechnology Laboratory, Center for Environmental Engineering Research, Department of Civil Engineering, The University of Hong Kong, Pokfulam Road, Hong Kong ...

Edited by Joseph Takahashi, University of Texas Southwestern Medical Center, Dallas, TX; received February 3, 2022; accepted June 7, 2022 ...

The obtained cDNA was diluted with ddH 2 O and stored in the refrigerator at -20°C for subsequent RT-qPCR experiments. (1) geNorm. When the geNorm algorithm is used to analyze the gene expression ...

This work was supported by the National Natural Science Foundation of China (32271174 and 32400955), the Key Program of National Social Science Fund of China (23AZD092), and the China Postdoctoral ...

The proposed system detects four positive animal-derived samples with a quantitation cycle (Cq) value of quantitative PCR (qPCR) in the range of 13 to 32. In addition, deep learning models are ...

Pipeline dedicated to finding the most stable genes among a single or multiple expression dataset(s). It is useful in particular for finding the most suitable RT-qPCR reference genes for a specific ...

Yet, biological evolution has provided us with visible signatures of social bonding—the neuropeptide oxytocin and its biobehavioural proxies—which may provide a starting point from which to ...

Therefore, this study successfully developed a highly efficient, rapid, specific, and sensitive multiplex qPCR method to detect major pathogens associated with bovine respiratory diseases. This ...

Reference genes are commonly used for normalization of target gene expression during RT-qPCR analysis. However, no housekeeping genes or reference genes have been identified to be stable across ...

The manuscript by de La Forest Divonne et al. offers an important and detailed exploration of the immune cells in the oyster Crassostrea gigas, by correlating distinct hemocyte morphotypes with ...