The GoTaq ® Probe 1-Step RT-qPCR System enables detection and relative quantification of RNA expression levels using a one-step RT-qPCR ... Promega's PCR Systems, enzymes and reagents are proven ...
Owing to extensive accumulation of primer-dimers when no template control is used during one-step RT-PCR, we introduced a two-step protocol to eliminate this problem. This study further ...
Although qRT-PCR studies ... amplification step improves the sensitivity of detecting even low-abundance transcripts from as little as one cell. Third, a single MessageBOOSTER kit reaction ...
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