The authors created a useful collection of Rep protein variants with changes that alter the amino acid sequence, but these did not lead to clear improvements in how the virus worked. Instead, their ...

From each mutant selection plate, we picked a random colony, plus any colonies of notable morphology, and streaked each onto LB agar plate and incubated at 37 °C overnight. We restreaked the colonies ...

Aliquots of 0.1 mL of the appropriate dilutions were inoculated, using spread plate technique, onto the surface of Petri dishes containing MRS agar, in duplicate. After incubation at 32ºC for 48 h ...

In nature, many animals respond to cold by entering hibernation, while in clinical settings, controlled cooling is used in transplantation and emergency medicine. However, the molecular mechanisms ...