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The well-studied NF-κB signaling system is a key mediator of the inflammatory response. Large-scale sequencing studies in ...

Fluorescence-based single-molecule and fluctuation spectroscopy in the near-IR can open avenues for biomolecular dynamic studies in biological media with suppressed autofluorescence and scattering ...

Super-resolution microscopy facilitates the visualization of cellular structures at a resolution approaching the molecular level. Especially, super-resolution techniques based on the localization of ...

Confocal Laser Scanning Microscopy and Image Analysis Confocal microscopy was done with a TCS SP5 Confocal Microscope fitted with a 63X water-immersion objective. GFP and FM4-64 were assayed using 488 ...

Image of a single molecule obtained with RASTMIN. c. ... In RASTMIN, only two main modifications to a confocal microscope need to be implemented. On the one hand, ...

Another technique, usually called “single molecule photobleaching” (“smPB”) has been shown to be an effective tool for determining the stoichiometry of small molecular aggregates (Gordon, Ha, and ...

Compared to classical confocal microscopy, ... Direct stochastic optical reconstruction microscopy utilizes the photoswitching of a single ... A., Buldt, G., and Gensch, T. (2020). Spatial filter and ...

The facility hosts three confocal microscopes and one Multiphoton (MP) confocal. These include the point scanning Leica TCS SP8 STED, the Leica SP8 DIVE MP the swept-field VT-iSIM and the Zeiss LSM700 ...

Three dimensional single-molecule tracking (3D-SMT) holds great promises of providing insights into molecular transport and localization dynamics in live cells. Sub-diffraction localization precision, ...

“While the microscope we describe is built for ALEX [alternating laser excitation] confocal smFRET, its modular design makes for easy expansion to several related techniques,” added the team. “Without ...