In vivo base editing enables precise, single-nucleotide changes to DNA without double-strand breaks. A recent application in ...

Precision gene editing is crucial for treating genetic diseases, as it enables targeted correction of specific mutations. A Korean research team has become the first in the world to significantly ...

Comparatively, prime editing relies on Cas9 nickase, a modified CRISPR-Cas9 system, fused to a reverse transcriptase enzyme capable of identifying and subsequently replacing specific DNA sequences ...

Tacked on to the Cas9 nickase is another enzyme called a DNA adenine deaminase. It chemically converts the DNA base adenine (A) to inosine (I).

In contrast, CREATE employs a Cas9 nickase in combination with the full-length, endonuclease inactivated ORF2p for targeted integration. Recent high-resolution cryo-EM studies that investigated ORF2p ...

The fusion construct and its interval of hypermutation could then be programmably targeted, through single-guide RNAs (sgRNAs), to specific genomic regions using a Cas9 nickase.

In this study, our research utilized the Cas9-NG nickase variant to minimize PAM sequence constraints, enabling the generation of paired nicks at desired genomic loci. We performed a systematic ...

The granted patent US12049651B2 introduces a novel Cas9 fusion molecule designed for gene editing applications. This molecule consists of an enzymatically active Cas9 protein covalently linked to a ...

In prime editing, the Cas9 nickase protein is guided to the target DNA sequence by the pegRNA. Once at the target site, the Cas9 nickase creates a single-strand nick in the DNA. The pegRNA contains a ...