In vivo base editing enables precise, single-nucleotide changes to DNA without double-strand breaks. A recent application in ...

Focal gene amplification is a common feature of advanced and metastatic cancers. Here, the authors employ CRISPR-Cas9 nickase to target cancer-associated focal gene amplifications to selectively ...

CRISPR-Cas9 genome editing exploits the CRISPR-Cas system to modify a genome in a targeted manner. Guided by RNA, the Cas9 endonuclease breaks DNA at a target sequence.