Both para-nitrophenylphosphate and p-nitrophenol are colorless at acidic pH values. Addition of 0.05 M NaOH after 30 min incubation at 37 °C will stop the reaction due to an increase in the pH of the ...
The pH of the solution was controlled using three different bases, which included tetramethylammonium hydroxide (TMAOH), sodium hydroxide (NaOH), and potassium hydroxide (KOH). The precipitated ...