An in vitro AAV transduction inhibition assay was used to quantify the amount of anti-AAV neutralizing serum in mouse and human serum. The serum supernatant was stored at −80°C and heat-inactivated at ...

Cell growth inhibition and apoptosis were tested using CellTiter-Glo (CTG, Promega) and Caspase-Glo 3/7 (Promega) assays according to the manufacturer’s instructions. HEK293T cells were grown in DMEM ...

College of Biological and Pharmaceutical Engineering, Anhui Engineering Laboratory for Conservation and Sustainable Utilization of Traditional Chinese Medicine Resources, Anhui Province Key Laboratory ...

Angiotensin-converting enzyme 2 binding inhibition assay Plasma samples were thawed, centrifuged for 3 min at 2,000×g at 4°C, and then diluted 1:100 in Diluent 100 buffer (Meso Scale Discovery, ...

Small Molecule Therapeutic Discovery, Amgen Research, Amgen Inc., One Amgen Center Drive, Thousand Oaks, California 91320, United States ...