Protease dependency assays indicated that both ... though their cleavage efficiency was lower than that of MERS-CoV. Trypsin ...

A total of 200 ug (400 uL) was transferred to a fresh 2 ml tube for trypsin digestion. Lyophilized trypsin protease (Pierce™ Trypsin Protease, MS Grade) was resuspended in 50 mM acetic acid to 0.5 ...

For the putative non-structural protein, the conserved domains of AstroV-HMU-1 detected by Pfam were trypsin-like peptidase domain (148–268), periplasmic serine protease (123–279), and RNA-dependent ...

Protein samples were sonicated three times on ice using a high intensity ultrasonic processor (Scientz) in lysis buffer (8 M urea, 1% protease inhibitor cocktail ... to urea concentrations < 2 M.

Department of Chemistry, Technical University of Darmstadt, Peter-Grünberg-Str. 8, Darmstadt 64287, Germany ...