PBS preparation. PC-12 cells (Shanghai Yiyan bio-technology Co., Ltd., China) are cultured as a monolayer of adherent cells in glass flasks under the following conditions: 37ËšC, saturated humidity ...
A non-irradiated culture was used as a control. The inactivation capacity of the different radiation doses was evaluated by subculturing serial dilutions of treated culture on TSA plates and ...
For short-term storage, the strains were maintained on inclined MRS agar slants at 4°C, with subculturing performed every 2 weeks to ensure their viability. For long-term preservation, the strains ...
Distribution of this vaccine to various countries, and more subculturing, led to genetic variations between different BCG strains. However, all strains possess a common deletion that occurred prior to ...
Sterility testing is a critical quality control measure that ensures pharmaceutical products and medical devices are free from microbial contamination before being released to patients. However, ...
Peer ReviewDownload a summary of the editorial decision process including editorial decision letters, reviewer comments and author responses to feedback. An evolutionarily conserved micropeptide, ...
Revised: This Reviewed Preprint has been revised by the authors in response to the previous round of peer review; the eLife assessment and the public reviews have been updated where necessary by the ...
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