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combining GoScriptâ„¢ Reverse Transcriptase and GoTaq ® Probe qPCR Master Mix in a single-step real-time amplification reaction. The GoScriptâ„¢ RT Mix for 1-Step RT-qPCR (50X) combines optimized ...
Real-time qPCR (RT-qPCR) was performed using the AB StepOnePlus Detection System (Applied Biosystems, Foster City, CA) using the one-step kit RT-PCR Master Mix Reagents (#64471423, Biorad), prepared ...
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