All MST experiments were performed on a Monolith NT.115 (Nanotemper, Germany) instrument and performed in MST buffer. The G4 DNA concentration was kept constant at 10–20 nM, to which GL-Os were ...
The thermal stability and unfolding of NA proteins were measured using the nanoDSF (differential scanning fluorimetry) technique on a Prometheus Panta instrument (Nanotemper). NanoDSF monitors changes ...