Pathway analysis, originally developed for gene expression data, has been adapted for metabolomics. However, owing to the unique characteristics and constraints of metabolites compared with genes ...

For CI in gel activity measurements, the BN-PAGE gel was incubated in 2 mM Tris/HCl pH 7.4, 0.1 mg/ml NADH (Roche), and 2.5 mg/ml nitrotetrazolium blue for blue staining (Sigma) for about 10 minutes.

Yusuf Hamied Department of Chemistry, University of Cambridge, Cambridge CB2 1EW, U.K.