the increased distance between the fluorophore and quencher in standard end-labeled probes may result in reduced quenching efficiency. In double-quenched BHQnova Probes the nova quencher is closer ...

(b) Before hybridization, the DNA probe is labeled by various means, such as nick translation, random primed labeling, and PCR. Two labeling strategies are commonly used: indirect labeling (left ...

FISH is a technique that involves fluorescently labeled probes to target specific chromosomal regions within DNA. In the context of cancer biomarker detection, these probes bind to DNA in tissue ...

Photo: Amy FrancisTerry Weber, research technician at the Children's Hospital of Philadelphia, uses radioisotopes to label probes for chromosomal mapping studies. Edwin M. Southern's 1975 paper, ...