In order to analyze the length of the PCR products by electrophoresis and a laser detection system, one of these primers has to carry a fluorescent dye label, which may be 6-carboxy-fluorescine ...

Endpoint analysis: Scientists analyze PCR products typically using agarose gel electrophoresis. Real-time PCR detects and quantifies DNA as the reaction progresses. This quantifies the specific amount ...

At the completion of the reaction, amplification products are analyzed by size fractionation using gel electrophoresis. Real-time PCR follows the same course, except that the products are detected ...

closed-tube system that allows for real time quantitative PCR without sample processing or electrophoresis. At the core of the system is a 5' nuclease assay conducted with the TaqMan¨ PCR Reagent Kit ...