This valuable manuscript investigates the localisation of nutrient receptors in bloodstream stage trypanosomes, with implications for both nutrient uptake and immune evasion. Results after direct ...

Results Expression and Extraction of Recombinant Amelogenin. Total protein was extracted from E. coli both immediately after the addition of IPTG and 1 h post induction to determine the presence of ...

The highly virulent and transmissible SARS-CoV-2 causes COVID-19 and poses a global public health threat. Herein cloned, expressed, and the molecular weight of the receptor-binding domain (RBD) of the ...

The results of Western Blot confirmed that the expression of TLR2, TLR4, and NF-κB protein in the synovium of Group M2 was higher than that of Group M1, whereas the expression of TLR2, TLR4, NF-κB, ...

A portion of thoracic aorta and the left carotid were frozen at −80°C for subsequent calcium, protein expression (Western blot), and CA activity (only for aorta) evaluation. Another portion of ...

Proinsulin was expressed by Escherichia coli SHuffle T7 with pET system in minimal medium containing glucose (Glu medium), glucose and glycerol (GluGly medium) or glycerol (Gly medium). With 100 ...

Among the hundreds of proteins that are secreted by Legionella pneumophila (the causative agent of Legionnaires’ disease) ...

Western blotting is a widely used technique for detecting and analysing specific proteins within cells or tissues. It allows confirmation of both the presence and molecular weight of target proteins, ...

We understand that gaining publication-quality results straight away is not the norm when performing western blot. This 32-page handy guide provides tips and tricks to obtain high-quality, publishable ...

Peer ReviewDownload a summary of the editorial decision process including editorial decision letters, reviewer comments and ...