Gene-editing tools such as CRISPR/Cas9 can be used to create isogenic cell lines, which can be further used to model a specific patient population. An isogenic cell line was created to model ...

Genome editing offers the potential to create new therapies and advanced cell-line models for pharmaceutical discovery and production. The combination of Cyto-Mine and CRISPR– Cas9 should enable ...

Ryotaro Hashizume and colleagues used the CRISPR-Cas9 gene editing system to cleave the third chromosome in previously ...

To validate this finding, they created a BORCS5 knockout cell line using CRISPR/Cas9 and confirmed, through rescue experiments, that this interaction is critical for lysosome transport.

When the CRISPR Cas9 protein is added to a cell along with a piece of guide RNA, the Cas9 protein hooks up with the guide RNA and then moves along the strands of DNA until it finds and binds to a ...

tested in human and mouse cells, rewrites DNA by only cutting a single strand to add, remove, or replace base pairs. The method may allow researchers to edit more types of genetic mutations than ...

How chromatin openness controls behavioral changes remains unclear. This work implicates alternative promoter selection, ...

As the demand for precision and efficiency in genetic research continues to grow, Creative Biogene's CRISPR library screening service stands out, allowing scientists to quickly identify and analyze ...

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2 BEs are fusion proteins made of a Cas9 enzyme that cleaves target DNA (nCas9 ... mutations in the FANCA gene in primary patient fibroblast and lymphoblastoid cell lines. Base editing restored FANCA ...

We are using Sendai viruses to reprogram blood cells. We can reprogram fibroblasts using CRISPR-Cas9-based gene activation (CRISPRa) or a conventional transgene reprogramming system. We guarantee the ...