Oxygenases have long been thought to require a cofactor for catalysis. The structure of a vancomycin biosynthetic enzyme in complex with a substrate analog, and with molecular oxygen bound in its ...

This enzyme has a unique metal cluster, termed the FeMo-cofactor, that requires eight electrons and eight protons to reduce N ...

The researchers used site-specific mutagenesis to gain new insights into how the cofactor precursor is integrated into the enzyme and how individual amino acids are involved in anchoring and ...