During ubiquitination, a group of enzymes called E3 ubiquitin ligases attach a small protein called ubiquitin to other proteins. This tagging, in turn, helps determine the fate of the targeted ...

Figure 5: Parkin exhibits ubiquitination activity in vitro. Parkin consists of two functionally different domains (Fig. 6): the C-terminal RING-box, which recruits a specific E2 enzyme (UbcH7 ...

Mass spectrometry has become the standard tool to map sites of ubiquitination, which is a covalent protein modification with a central role in regulating cellular processes 1. Ubiquitin is a small ...