This also means that DNA-PAINT can use a large pool of existing fluorophores that were previously not applicable to localization-based super-resolution microscopy. Although DNA-PAINT offers ...

Previous fluorescent markers for target proteins have been too large and this has been a key factor slowing down the progression of super-resolution microscopy. Therefore, the team decided to downsize ...

In this study, the researchers applied cutting-edge super-resolution microscopy that enabled them to visualize the protein scaffold as it assembled around the DNA break. “This could be compared to ...

including stimulated emission depletion (STED), localization-based super-resolution and diffraction-limited microscopy. Figure 1: DNA origami standards for fluorescence microscopy. DNA origami ...

Although super-resolution microscopy is cited in a growing number ... and the formation of molecular assemblies such as DNA repair complexes, ribosomes, protein aggregates, and synaptic scaffolds.

More information: Jonas Zähringer et al, Combining pMINFLUX, graphene energy transfer and DNA-PAINT for nanometer precise 3D super-resolution microscopy, Light: Science & Applications (2023).

This instrument supports multiplexing applications such as DNA-PAINT and spatial multiomics imaging. Bruker’s Vutara super-resolution microscopy product manager, Winfried Wiegraebe, PhD ...

DNA is organized as chromatin ... nucleosomes during cell division in living human cells using super-resolution microscope," said Kazuhiro Maeshima, professor at the National Institute of Genetics ...

By labeling adjacent targets, too close to each other to be resolved even by super-resolution microscopy, with different DNA strands, an additional degree of differentiation (a barcode) is introduced ...

Researchers have now developed DNA-PAINT, a variant of these so-called super-resolution approaches. Recent advances in fluorescence microscopy allow researchers to study biological processes below ...

Recent advances in fluorescence microscopy allow researchers ... a variant of these so-called super-resolution approaches. "DNA-PAINT yields super-resolved images using comparably simple ...

Recent advances in fluorescence microscopy allow researchers to study biological ... a variant of these so-called super-resolution approaches. “DNA-PAINT yields super-resolved images using comparably ...