This also means that DNA-PAINT can use a large pool of existing fluorophores that were previously not applicable to localization-based super-resolution microscopy. Although DNA-PAINT offers ...

Previous fluorescent markers for target proteins have been too large and this has been a key factor slowing down the progression of super-resolution microscopy. Therefore, the team decided to downsize ...

More information: Jonas Zähringer et al, Combining pMINFLUX, graphene energy transfer and DNA-PAINT for nanometer precise 3D super-resolution microscopy, Light: Science & Applications (2023).

Although super-resolution microscopy is cited in a growing number ... and the formation of molecular assemblies such as DNA repair complexes, ribosomes, protein aggregates, and synaptic scaffolds.

In this study, the researchers applied cutting-edge super-resolution microscopy that enabled them to visualize the protein scaffold as it assembled around the DNA break. “This could be compared to ...

This instrument supports multiplexing applications such as DNA-PAINT and spatial multiomics imaging. Bruker’s Vutara super-resolution microscopy product manager, Winfried Wiegraebe, PhD ...

Super-resolution microscopy enables scientists to look beyond the limit ... Moreover, PAINT uses transient fluorophore-target binding through DNA pairs, enabling the detection of multiple targets in ...

DNA is organized as chromatin ... nucleosomes during cell division in living human cells using super-resolution microscope," said Kazuhiro Maeshima, professor at the National Institute of Genetics ...

By labeling adjacent targets, too close to each other to be resolved even by super-resolution microscopy, with different DNA strands, an additional degree of differentiation (a barcode) is introduced ...

Researchers have now developed DNA-PAINT, a variant of these so-called super-resolution approaches. Recent advances in fluorescence microscopy allow researchers to study biological processes below ...

Recent advances in fluorescence microscopy allow researchers ... a variant of these so-called super-resolution approaches. "DNA-PAINT yields super-resolved images using comparably simple ...

Recent advances in fluorescence microscopy allow researchers to study biological ... a variant of these so-called super-resolution approaches. “DNA-PAINT yields super-resolved images using comparably ...