In living organisms, protein molecules with different chemical structures called proteoforms, are produced from a single gene ...
MS, an experimental protocol combining high-resolution protein fractionation using SDS-polyacrylamide gel electrophoresis (SDS-PAGE) with the latest mass spectrometry technology to enable highly ...
to prepare the proteins for SDS-PAGE. The method described here 1 uses carrier ampholytes to form a pH gradient in a long, thin (1.2-mm) focusing gel composed of a low percentage (2.7% ...