Using the structure of this mutant as the search model ... For MfPC with B-domain deletion, the protein was incubated with 2.5 mM pyruvate for 30 min at 4 °C before crystallization set ...

Two mitochondrial enzymes -- pyruvate carboxylase (PC) and glutaminase replenish carbon to the Krebs cycle. A new study by University of Kentucky Markey Cancer Center researchers suggests that ...