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Cell division occurs through a series of checks and balances known as the cell cycle ... propidium iodide (PI). This dye will bind DNA, and during flow cytometry, the DNA will emit a fluorescent ...
The cell cycle is a target for many ... by staining the nuclei of fixed cells with propidium iodide. The cells are then sorted by flow cytometry into G1, S and G2/M populations according to ...
The fluorescence intensity ... to determine the cell cycle stage of cells on slides or in culture. Flow cytometry may be used to analyse cell cycle in live cells by loading cells with Hoechst 33342 at ...
The determination of the cell cycle analysis is a well known technique in flow cytometry. The Core Facilities at ICMS ... Jurkat cells stained with DNA binding dyes such as propidium iodide (PI) or ...
Flow cytometry is ... to mark out these cells. Propidium iodide is one such DNA-binding dye, which can also be used to differentiate between the stage of the cell cycle the cell is currently ...
The Flow Cytometry and Cell Separation Facility provides advanced cell sorting and cell analysis techniques including immunofluorescence detection of 18 colors, cell-cycle distribution, apoptosis ...