Adjust the pH to 4.5 for buffer A and to 6.5 for buffer ... performed in artificial cytoplasm or in situ. Perform the calibration procedure described in Box 4. Plate cells onto eight-well Ibidi ...

Figure 2: ClopHensor pH and [Cl −] i calibration in live cells ... for cellular regions. This procedure easily discriminated the regions of interest and prevented artifacts arising from dividing ...