However, at high temperatures the rate decreases again because the enzyme becomes denatured and can no longer function. This is shown in the graph below. In the graph above, as the pH increases so ...

As the temperature increases, the rate of enzyme activity increases, up to 40°C, which is the optimum temperature for the enzyme. Beyond 40°C, the enzyme becomes denatured.

Template fragments are generated by: (1) digestion of genomic DNA with a combination of the two restriction enzymes EcoRI and ... and the display on denaturing polyacrylamide gels of the EcoRI ...