Indeed, a mixture of CultrexBME/Matrigel and SCLC cells induced tumor growth from as few as 2.5 × 10 4 cells, which otherwise were unable to generate detectable tumors.

Matrigel ® containing single cells or organoid fragments is pipetted into the center of a well in a 24-well or 48-well tissue culture treated plate to form a dome (10 μL in 48-well plate, 30 μL ...

In contrast, Matrigel did not support stromal cultures and had partly disintegrated and shrunken by day 15. The design of the synthetic ECM also allows cells to sequester their own matrix as they ...

Organoids—self-organizing, self-reproducing structures that serve as 3D in vitro models—date back to 2009. Back then, researchers at the Hubrecht Institute led by Hans Clevers, PhD, introduced ...

An optimized method for RNA extraction from highly invasive glioma cells embedded into Matrigel was achieved by using TRIzol, used for matrix dissociation, cell lysis and RNA separation, in ...

This application note describes the use of the Incucyte® Live-Cell Analysis System along with the Incucyte® Organoid Analysis Software Module to study the growth or death of organoids, label-free. A ...

Researchers developed a method to produce artificially grown miniature brains -- called human brain organoids -- free of animal cells that could greatly improve the way neurodegenerative ...

Since highly versatile human stem cells were discovered at the University of Wisconsin-Madison nearly 20 years ago, their path to the market and clinic has been slowed by a range of complications.

hESCs grown on Matrigel in defined culture media. The mesh-like structure in the background is Matrigel. Disclaimer: AAAS and EurekAlert! are not responsible for the accuracy of news releases ...