Biotinidase (E.C. 3.5.1.12) is a 70–80 kD glycoprotein that is ubiquitously expressed and detectable in multiple sample types including serum, leukocytes, fibroblasts, and liver. 5 The enzyme ...

Assay of pyruvate carboxylase (another biotin enzyme) is included as an internal control. Two confluent T25 flasks filled to the neck with media in a non-vented flask and tape/parafilm shut. If ...