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Adeno-associated virus ... cells) or neurons (NeuN-positive cells) were evaluated, and, among each of those populations, the GFP-positive cells. Only glial and neuronal cells with DAPI-positive ...
Tissue culture ... cells were overlayed with full growth media 30 min later and incubated for 48 h (37°C, 5% CO 2). The cells were analysed for GFP expression by fluorescence microscopy. AAV ...
Labeling of CA1 excitatory neurons using the AAV-MG1.2 capsid. Excitatory neurons appear in green, cell nuclei are stained with DAPI (blue), inhibitory neurons are marked in cyan, and astrocytes ...
Adeno virus vector and Adeno-associated virus (AAV) vector-based vaccines have all played a key role in controlling the recent COVID-19 pandemic. Especially cell culture produced AAV vectors have ...